Crystallization and preliminary X-ray analysis of a C-terminal TonB fragment from Escherichia coli.
نویسندگان
چکیده
The TonB protein located in the cell wall of Gram-negative bacteria mediates the proton motive force from the cytoplasmic membrane to specific outer membrane transporters. A C-terminal fragment of TonB from Escherichia coli consisting of amino-acid residues 147-239 (TonB-92) has been purified and crystallized. Crystals grew in space group P2(1) to dimensions of about 1.0 x 0.12 x 0.12 mm. A native data set has been obtained to 1.09 A resolution.
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عنوان ژورنال:
- Acta crystallographica. Section D, Biological crystallography
دوره 60 Pt 7 شماره
صفحات -
تاریخ انتشار 2004